Guidelines Aim to Reduce Infections After Surgery by 70%

A team of researchers in University of Iowa has recommended guidelines that will cut the infection rate by 71 percent for staph bacteria and 59 percent for a broader class of infectious agents known as gram-positive bacteria.

The paper describing the guidelines has been published on June 13 in the British Medical Journal.

The researchers recommend three steps to reduce post-surgical staph infections:

• Swab patients’ noses for two strains of staph (MRSA and MSSA) before surgery
• Patients who have staph naturally in their noses, apply a anti-bacterial nose ointment in the days before surgery
• At surgery, give an antibiotic specifically for MRSA to patients who have the MRSA strain in their noses; for all others, give a more general antibiotic

Staph infections in hospitals are a serious concern and up to 85 percent of staph infections after surgery are descibed as to come from patients’ own bacteria.

Schweizer and colleagues conducted this study that included review of  39 studies of various surgical-site infection practices employed at hospitals nationwide.

Source

  1. Be Gone, Bacteria. Newswise
  2. M. Schweizer, E. Perencevich, J. McDanel, J. Carson, M. Formanek, J. Hafner, B. Braun, L. Herwaldt. Effectiveness of a bundled intervention of decolonization and prophylaxis to decrease Gram positive surgical site infections after cardiac or orthopedic surgery: systematic review and meta-analysis. BMJ, 2013; 346 (jun13 1): f2743 DOI: 10.1136/bmj.f2743

Xray of Lytic Lesion in Scaphoid Bone

Following xray of adult wrist showing a lytic lesion in the scaphoid

Lytic Lesion Scaphoid

Lytic Lesion Scaphoid

The arrow points to a well demarcated lytic lesion which as per records is suggested to be of infective origin. The patient appears to be lost in follow up and hence complete information is not available.

Xray of Tuberculous Arthritis Hip

Xray of tuberculous arthritis of hip in an adult patient.

Tuberculous Arthritis Hip

Tuberculous Arthritis Hip

Arthritis is suggested by reduced space in the joint and sclerosis [icreased radiodensity]  on acetabular margins.

Xray of Osteomyelitis Tibia With Bowing of Tibia Following Tibial Fracture

45 years old male patient underwent plate fixation for fracture tibia in lower third region. The plate got infected and was removed and tibia was fixed with external fixator.

The fracture united eventually and the fixator was removed.

Following xray is after 5 years of surgery when patient presented with discharging sinus.

Osteomylities of Tibia With Bowed Tibia

Osteomyelities of Tibia With Bowed Tibia

The xray shows a bowed tibia with irregularly thickened shaft and multiple lytic lesion suggestive of cavities. There is also a defect in tibial cortex anteriorly which is remnant of saucerization surgery done two years back for osteomyelitis.

An analysis of previous xrays showed that bowing was result of malunion following fixator application.

A broken Schanz pin is also visible

Patient was advised saucerization again but he refused.

Nanoparticles Deliver Higher Dose of Antibiotics to Sites of Infection

A study reported in ACS Nano journal has reported development of nanoparticles capable of delivering higher antibiotic doses to sitexs of infection.

The study has been published on web on April 3, 2012 and is reported by Aleksandar F. Radovic-Moreno and colleagues

It si a well known fact that bacteriae have remarkable capacities to develop resistance to antibiotics. Some drugs may be effective at higher doses but the side effects of the drugs prohibit from administering higher doses to the patients.

To overcome this limitation researchers at Massachusetts institute of technologyand Brigham and Women’s Hospital have developed a nanoparticle that can deliver large doses of antibiotics right to the site of bacterial infection. [Read more...]

Fluorescin In Situ Hybridization (FISH)

Fluorescence in situ hybridization is a cytogenetic technique that is used to detect and localize the presence or absence of specific DNA sequences on chromosomes.

FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence complementarity.

Fluorescence microscopy can be used to find out where the fluorescent probe bound to the chromosomes.

Uses

Fluorescence in situ hybridization  is used in genetic counselling, medicine, and species identification.

It can also be used to detect and localize specific mRNAs within tissue samples and thus identify organisms  which otherwise are not identifiable on routine tests.

 

Further resources on FISH

Enzyme linked Immuno Sorbent Assay (ELISA)

All microbial species have one antigen which is unique. These antigens can be purified and used to generate specific monoclonal antibodies.
Both the things, antigen and antibodies provide a highly specific diagnostic tool.

The term ELISA stands for enzyme linked immunosorbent assay is a serological test to look for presence of particular antigen or antibody.

There are two types of assays in ELISA

Direct

Direct ELISA uses monoclonal antibodies to detect the antigen. [Read more...]

Polymerase chain reaction (PCR)

The polymerase chain reaction (PCR) is a technique in molecular biology to amplify a single or a few copies of a piece of DNA to produce thousands to millions of copies of a particular DNA sequence within short time.

The technique was developed in 1983 by Kary Mullis.

A typical PCR involves 20-40 repeated temperature changes, called cycles, with each cycle commonly consisting of 2-3 discrete temperature steps.

Initialization step

This step consists of heating the reaction to a temperature of 94–96 °C . It is required for activation of DNA polymerase.

Denaturation Steps

 

This step consists of heating the reaction to 94–98 °C for 20–30 seconds. It causes DNA melting of the DNA template . [Read more...]

Overview of An Infected Implant and Its Management

An infected implant is not only a failure of surgical goal but also results in increased morbidity, prlongs the treatment and can affect the outcomes of treatment. It is deemed as most devastating complication of surgery.

Any implant can get infected and when it does, the infection is quite stubborn to go. The main reasons of persistent infection is biofilm, a kind of microcolony or cell cluster. The bacteriae in the biofilm are resistant to the treatment because they are irreversibly attached to each other and bathe in an extracellular matrix. This gives them extra protection from host.

Moreover, traditional investigations may not be able to grow the bacteriae and thus detect the infection because biofilms do not grow on agar plate when recovered by scrapping.

Antibiotic therapy can control the acute episodes but cannot  the biofilms per se.

An infected implant is a challenge to recognize, investigate and treat.

It is also important to recognize early subtle signs so that early measures can be taken.

[Read more...]

Chronic Non Healing Ulcer On The Leg With Underlying Bone Exposed

The following clinical photograph shows a chronic non healing ulcer of the leg. Underlying bone is exposed too.

Non Healing Ulcer On The Leg With Underlying Bone Exposed

Non Healing Ulcer On The Leg With Underlying Bone Exposed

Discoloration around the ulcer shows chronic nature of the lesion.

Note: The management of such lesions include control of infection, debridement [removal of dead tissue including bone], covering the wound with a suitable flap with or without fixation [as needed].