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Fluorescin In Situ Hybridization (FISH)

January 21, 2012 by 1 Comment

Fluorescence in situ hybridization is a cytogenetic technique that is used to detect and localize the presence or absence of specific DNA sequences on chromosomes.

FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence complementarity.

Fluorescence microscopy can be used to find out where the fluorescent probe bound to the chromosomes.

Uses

Fluorescence in situ hybridization  is used in genetic counselling, medicine, and species identification.

It can also be used to detect and localize specific mRNAs within tissue samples and thus identify organisms  which otherwise are not identifiable on routine tests.

 

Further resources on FISH

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  1. Implant Infections In Musculoskeletal System | Bone and Spine says:
    February 14, 2012 at 10:53 am

    [...] methods like Polymerase chain reaction (PCR), Fluorescin In Situ Hybridization (FISH), Enzyme linked Immunno Sorbent Assay (ELISA) may help to detect the [...]

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