Amyloid stain
Staining for amyloid is one of the most useful special techniques. The Congo red stain and examination with nonpolarized and polarized light are the most frequently used techniques. The dye binds the beta-pleated arrangement of amyloid and has no chemical specificity.
This is seen as a reddish deposit under nonpolarized light. Green birefringence is present when the sections are examined under polarized light. Excessive or prolonged exposure to Congo red can cause binding to the tissue that is unrelated to the presence of amyloid.
Hemosiderin
The most frequently used stain for hemosiderin is an iron stain. In this technique the acid hydrolysis separates the iron from the protein. Potassium ferrocyanide is subsequently used and forms an insoluble ferric ferrocyanide also known as Prussian blue.
Melanin stain
The most frequently used Fontana-Masson technique is a silver stain without an external reducing substance (argentaffin reaction). In this technique, metallic silver is directly reduced by melanin..
Calcium stain
Von Kossa’s technique for calcium is another variant of a silver stain. Calcified tissue such as mineralized osteoid is identified by the substitution of calcium with potassium and is reduced to an insoluble black silver precipitate with the photographic developer. Von Kossa’s technique is frequently used on nondeclacified bone sections for histomorphometric assessment of metabolic bone disorders.
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Hi really enjoyed reading your post.
I am having soreness on my spine (on bone) and it is very sensitive to touch. Any ideas what this might be?
Dr Arun Pal Singh Reply:
August 8th, 2010 at 10:19 am
@Laura
That is too little information for deducing.
sir, please send me calcium blue special stain procedure and preparation result. known. please.
THANK U SIR,
Dr Arun Pal Singh Reply:
July 31st, 2011 at 4:09 pm
@shaik moin ahmed,
You need to ask the concerned specialist.