Histomorphometry represents a microscopic planimetry or stereology. It is used to study homeostasis of the skeleton, mainly in metabolic disorders of bone. The technique is occasionally used to evaluate the skeletal status and treatment effect of rickets and osteomalacia associated with tumors.
For histomorphometry, undecalcified bone sections stained with techniques that enable the visualization of calcified and uncalcified osteoid such as von Kossa’s or Goldner’s (modified trichrome) stains are used. Another frequently used technique is tetracycline-pulse labeling for epifluorescence. [Read more...]